A USB drive or SD card can hold more than files: it can carry a boot sector, hidden partitions, and a layout that a plain file copy misses. To preserve it exactly, capture the whole device as a single image file, which can later be written back to another stick or card byte for byte.
This guide explains the difference between an ISO and a raw image, which tools capture the full device, and how to write the image back when you need it.
For a USB drive, a raw image is almost always the right choice. It preserves the boot sector, the partition table, and every hidden area that a file copy would leave behind.

The goal is the same whatever the tool: read the entire block device into one file. The file is then an exact snapshot of the USB as it was at that moment.

On Linux, the command is typically dd if=/dev/sdX of=usb-backup.img bs=4M status=progress, where sdX is the USB device. On Windows, Win32 Disk Imager picks the drive and writes the file in a few clicks.
An image file is useful only if it can be restored. Writing it back to another USB stick or SD card rebuilds the original device exactly, including the ability to boot.
Watch the video tutorial below to clone the drive step by step with Qiling Disk Master.
Step 1. Open Qiling Disk Master, choose Clone, and then select Disk Clone.

Step 2. Select the USB drive as the source and click Next.

Step 3. Select a destination disk or an image file and confirm that its data will be replaced.

Step 4. Keep every partition, and confirm that the boot sector is part of the copy.

Step 5. Click Proceed, wait for the copy to finish, then safely eject the new device.

A raw image of an empty 32 GB stick is still 32 GB. Compression removes most of that empty space, but the image must be expanded again before it is written back.
No. An ISO represents an optical file system, while a raw image captures every sector of the device, including the boot code.
Not for a bootable device. A file copy misses the boot sector and hidden partitions, so the copy will not start.
Use dd with the device path, for example dd if=/dev/sdb of=usb.img bs=4M status=progress.
Only if the image is shrunk first. A raw image fits exactly on a drive of the same size or larger.
Yes. Qiling's disk backup reads the whole device, so the boot code and partition table are included.
Capturing a USB drive as an image carries risk, and a bad write can corrupt the original. Create a full backup first, so a mistake costs nothing but time.
Watch the video tutorial below to back up Windows and restore it if the clone fails.
Step 1. Open Qiling Disk Master and select Disk Backup to include every partition and the boot sector.

Step 2. Save the image to a drive other than the source USB and start the backup.

Step 3. Click Proceed to start the system backup and wait patiently until it finishes. You can also tick Shut down the computer when the operation is completed to power off automatically.

Step 4. If the image fails to restore, use the Qiling backup to rebuild the USB on another stick.

A USB drive should be captured as a raw image, not an ISO, because only a sector copy preserves the boot code and the full partition layout. Use dd on Linux, Win32 Disk Imager on Windows, or Qiling Disk Master for a backup that can restore the device exactly. Verify the image, compress it for storage, and keep the original until the restored device boots.
For more on full-disk backups, see How to Clone a Raspberry Pi SD Card, How to Clone a Hard Drive to a Smaller SSD, Does Cloning a Hard Drive Copy Windows, and How to Migrate OS to SSD Without Reinstalling Windows.