Changing the motherboard replaces the part of the PC that Windows knows best. A cloned drive can still boot on the new board, but the drivers, the boot mode, and the activation all need attention, so the upgrade should start with a reliable image of the current system.
This guide explains how to clone the drive, prepare Windows for new hardware with Sysprep, and repair the boot if the first start on the new board fails.
A clone is a good start, but it is not the whole job. The system has to be prepared for new hardware, or the first boot can hang on a black screen or a boot device error.

The more the new board differs from the old one, the more a clean install pays off. For a same-vendor upgrade from one generation to the next, a clone often works with a little driver cleanup.

Sysprep generalizes the installation so Windows redetects the hardware. Without it, the cloned system may keep trying to load drivers for a chipset that no longer exists.
Qiling Disk Master copies the whole system disk, including the EFI and recovery partitions, to another drive or to an image file. The destination is overwritten, so confirm it holds nothing you still need.
Watch the video tutorial below to clone the drive step by step with Qiling Disk Master.
Step 1. Open Qiling Disk Master, choose Clone, and then select Disk Clone.

Step 2. Select the current system disk as the source and click Next.

Step 3. Select the destination disk or image file and confirm that its data will be replaced.

Step 4. Keep the EFI and recovery partitions, and confirm the boot mode matches the new motherboard.

Step 5. Click Proceed, wait for the copy to finish, then shut the machine down before you swap the board.

If Windows stops on a boot device error, the boot mode usually does not match the disk style. Switch the firmware between UEFI and Legacy, or repair the boot files from installation media.
Often yes, but Windows may need new drivers and reactivation, and the boot mode must match the new firmware.
It is recommended. Sysprep removes old drivers so Windows redetects the new hardware cleanly.
Most do, but software locked to the old hardware may need reactivation or reinstallation.
The boot mode or storage controller mode usually does not match. Set the firmware to AHCI and to the correct partition style.
A clean install is more reliable on very different hardware, but a clone saves the time of reinstalling everything.
A motherboard swap is one of the riskiest hardware changes for Windows. Create a full backup before the clone, so a failed boot costs nothing but time.
Watch the video tutorial below to back up Windows and restore it if the clone fails.
Step 1. Open Qiling Disk Master and select System Backup to include the boot partitions.

Step 2. Save the image to a drive other than the target disk and start the backup.

Step 3. Click Proceed to start the system backup and wait patiently until it finishes. You can also tick Shut down the computer when the operation is completed to power off automatically.

Step 4. If the new board will not start, restore the image to the original drive and boot it on the old hardware.

A motherboard swap needs more than a drive clone. Generalize Windows with Sysprep, match the boot mode to the new firmware, keep the storage controller in AHCI, and have a backup ready. Then let Windows redetect the hardware, install the chipset drivers, and reactivate if the licence does not follow.
For more on hardware changes, see How to Migrate Windows 10 from One PC to Another, Windows Not Activated After Cloning, How to Clone Windows 11 to a New Laptop, and How to Clone a Windows Server System Disk.