A Raspberry Pi runs from an SD card that is written to constantly and wears out faster than a normal disk. Keeping a tested copy means a card failure costs a reboot instead of a rebuild.
This guide explains how the Pi card is laid out, which details matter when you duplicate it, and how to boot the copy on the same board.
A clone is a working duplicate, not a backup file. Keep one card running and one card stored, and refresh the stored copy after major changes.

Because the root volume is not readable by Windows, the copy must be made at disk level. A file copy from Explorer cannot see ext4 data at all.

Keep the source card untouched until the copy has booted. A card that only reads is much harder to damage than one that is still in service.
Qiling Disk Master can copy a removable card at disk level, including the small boot volume, the Linux partitions, and the gaps between them. Download it on a Windows PC with a card reader.
Step 1. Open Qiling Disk Master, choose Clone, and then select Disk Clone.

Step 2. Select the Raspberry Pi card as the source and click Next.

Step 3. Select the second card as the destination and confirm that its data will be replaced.

Step 4. Keep every partition and the original size, then review the plan before you continue.

Step 5. Click Proceed, wait for the task to finish, then use Safely Remove Hardware before taking the cards out.

Keep the original card until the copy has run for a week. If the copy refuses to start, the boot-partition checks in Cloned Drive Wont Boot Fix point at the usual causes.
Yes. A disk-level clone copies the raw blocks, so Windows does not need to read the ext4 volumes.
Equal or larger is safest. A larger card works, but the root partition must be expanded before the extra space is usable.
Only one at a time, unless you change the hostname and IP address on the second card first.
Only when the used data fits. Shrinking needs a Linux tool that resizes the file system first, which a plain clone does not do.
After every significant configuration change, and at least once a season for a board that runs continuously.
Before changing drives or partitions, create a full system backup. Qiling Disk Master creates an image you can restore from.
Step 1. Open Qiling Disk Master and select System Backup to include Windows and the boot partitions.

Step 2. Save the image to a drive other than the card reader and start the backup.

Step 3. Click Proceed to start the system backup and wait patiently until it finishes. You can also tick Shut down the computer when the operation is completed to power off automatically.

Step 4. If a card is wiped by mistake, restore the Windows image and rebuild the Pi media from the spare card.

A spare SD card turns a Raspberry Pi failure into a two-minute swap. Shut the board down cleanly, use a reliable reader, and pick a card that is at least as large as the source. Clone at disk level with Qiling Disk Master so the boot volume, the ext4 root, and the gaps between them all travel together. Then boot the copy, change the hostname and IP, expand the file system on a larger card, and keep the original until the copy has proven stable.
For more on removable media, see How to Clone a Bootable USB Drive to Another USB, How to Clone a Navigation SD Card, Cloned Drive Wont Boot Fix, and How to Clone a Hard Drive to a Larger Hard Drive.