A desktop board with a single SATA header leaves no obvious place for a second drive. Cloning normally needs the source and the target attached at the same time, which is exactly what one port cannot offer. The workaround is to attach the new drive through USB and copy while Windows still runs from the old one.
This guide covers the USB bridge options, the limits of each, and how to finish the copy without opening the case twice.
The source stays on the board's SATA port, the target hangs off USB, and the clone runs from the live system. No reboot into a rescue tool is required.

Connect the adapter, wait for Windows to list the disk, and note its capacity. If nothing appears, try another port before blaming the drive.

Label the two drives before you start. Selecting the wrong one erases the system you meant to keep.
Qiling Disk Master lists the SATA drive and the USB target side by side. The steps match a two-port setup, and the software handles the boot files for the new disk.
Watch the video tutorial below to clone the drive step by step with Qiling Disk Master.
Step 1. Open Qiling Disk Master, choose Clone, and then select Disk Clone.

Step 2. Select the source disk and click Next.

Step 3. Select the destination SSD and confirm that its data will be replaced.

Step 4. Choose the clone mode and confirm the system partition gets the C letter on the target.

Step 5. Click Proceed, wait for the copy to finish, then shut the machine down before you remove any cable.

If the system does not start, the clone probably missed the boot partition. Re-clone with every partition selected and verify the boot files again.
Yes. Connect the target through a USB adapter or dock and clone from the running system.
A USB 3.0 bridge reaches 5 Gbps, which is close to SATA III speed in practice.
Only for 3.5-inch drives, which draw more current than a USB port supplies.
Yes, as long as the boot and system partitions are included in the clone.
Try a rear port, a shorter cable, or another enclosure before assuming the drive is faulty.
A clone through a USB bridge is more fragile than an internal copy. Take a system image first so the old drive can be restored if the adapter fails.
Watch the video tutorial below to back up Windows and restore it if the copy fails.
Step 1. Open Qiling Disk Master and select System Backup to include the boot partitions.

Step 2. Save the image to a drive other than the target disk and start the backup.

Step 3. Click Proceed to start the system backup and wait patiently until it finishes. You can also tick Shut down the computer when the operation is completed to power off automatically.

Step 4. If the clone or the boot repair fails, restore the image and then reassign drive letters or rebuild the boot records from Windows Recovery.

One SATA port is not a dead end. Put the target on USB, keep the bridge powered and steady, and confirm the copy boots before you move the new drive onto the board. The single port then serves the SSD exactly as it served the old disk.
For more on USB-based cloning, see Clone an HDD to an SSD With a USB Cable Only, Clone a Drive With a Docking Station, Clone a Drive With an NVMe Enclosure, and Clone a SATA SSD to an NVMe SSD.