A desktop with a single M.2 slot can still gain a second NVMe drive through a PCIe adapter card. It is a cheap alternative to an enclosure, and it keeps both drives inside the case at full speed.
The catch is that slot choice and firmware support decide whether the new drive only stores data or can also boot. This guide covers both cases.
Check your motherboard manual for the lane layout, because two slots often share bandwidth and installing a card can slow another device.

If the drive does not appear, try another slot before you assume the card is faulty, since shared lanes and disabled slots are common.

Test the new boot with the old drive still connected but lower in the boot order, so you can fall back without opening the case again.
Qiling Disk Master sees both the board slot and the adapter drive as normal disks, so it can clone the system, resize the target and align it for the SSD. It also handles the backup step at the end.
Watch the video tutorial below to clone the drive step by step with Qiling Disk Master.
Step 1. Open Qiling Disk Master, choose Clone, and then select Disk Clone.

Step 2. Select the source disk and click Next.

Step 3. Select the destination SSD and confirm that its data will be replaced.

Step 4. Choose the clone mode and adjust the partition size if the target is larger.

Step 5. Click Proceed, wait for the copy to finish, then shut the machine down before you remove any cable.

Decide early whether the adapter drive is storage only or the new system disk, because that choice determines how much firmware work the project needs.
Often yes on modern boards with NVMe boot support, but older boards may need a firmware update or can boot from the adapter at all. Check the manual and test before you erase the old drive.
Only if the card or slot has fewer lanes or an older generation than the drive needs. A single-drive x4 card in a 4.0 x4 slot can run the drive close to its rated speed.
No. A single-drive card uses the whole slot as one link. Bifurcation matters only when one card carries several M.2 drives.
Check the seating, the slot lane sharing and the firmware setting for the slot. Some boards disable a slot when another device uses the same lanes.
Yes. Keep it as a fallback, then remove or wipe it once the adapter drive has booted several times without problems.
An adapter adds a card, a slot and a firmware setting to the equation, and a clone that fails halfway can leave the system unbootable. Keep a system image on a separate disk so a bad slot or card costs you time, not data.
Watch the video tutorial below to back up Windows and restore it if the clone fails.
Step 1. Open Qiling Disk Master and select System Backup to include the boot partitions.

Step 2. Save the image to a drive other than the target disk and start the backup.

Step 3. Click Proceed to start the system backup and wait patiently until it finishes. You can also tick Shut down the computer when the operation is completed to power off automatically.

Step 4. If the clone fails, restore the image and check the source disk before you retry.

A PCIe adapter is a practical way to add a second NVMe drive to a desktop with one M.2 slot. Verify the lane width, the generation and the boot support first, and keep the original drive installed until the adapter drive has started Windows on its own.
For related guides, see Clone a SATA SSD to an NVMe SSD, Clone to a PCIe 5 SSD, Clone a Boot Drive with Multiple EFI Partitions, and 4K Alignment When Cloning an SSD.